Electrophoresis of Glutamic Oxalacetic Transaminase in Serum, Beef Heart, and Liver Homogenates on Cellulose Acetate.

‘THE RECENT DEVELOPMENT of a new colorimetric procedure (TransAc*), for the determination of serum glutamic oxalacetic transaminase utilizing the diazonium salt, 6-benzamido-4-methoxy-m-toluidine diazonium chloride, as the color developer with a specificity for oxalacetic acid, prompted the following experiments. Determination of glutamic oxalacetic transaminase activities were performed according to the method of Babson et al. (1) on beef heart homogenate, beef liver homogenate, and sera containing glutamic oxalacetic transaminase in the normal and the abnormal ranges. These specimens were then subjected to electrophoresis on cellulose polyacetate strips. The color of the bands were then developed by means of the buffered substrate and color developer used for the colorimetric procedure.